Structure–Effect Relationships of Novel Semi-Synthetic Cannabinoid Derivatives
Marcus R | Juan A | Fernández-Ruiz | Bernd L | García-Toscano | Gómez-Cañas | Maria R
The membranes were incubated for 90 min at 30°C with the radioligand and different concentrations of the different derivatives. Anti-Inflammatory Effect on Human Monocytes To test the anti-inflammatory effect of the test compounds, monocytes were incubated for 30 min with increasing doses of the test compounds. Trend tests were performed using the Jonckheere–Terpstra test. The CBD-derivatives 2-HEC (1), 2-HPC (2), GCBD (3), CHC (4), and NMSC (6) activated the CB2-receptor and acted as agonists (Figures 1 and 4). 2-HEC (1), 2-HPC (2), GCBD (3), and NMSC (6) significantly inhibited PMA/IO-induced NFAT activation of Jurkat T-cells (Figure 6) in a dose-dependent fashion (trend tests: p = 0.038, p = 0.005, p = 0.006, and p = 0.06, respectively).