Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression
To further verify the role of MGLL in lipid accumulation in TAMs, we constructed a transgenic mouse model with specific overexpression of MGLL (TgMGLL) in myeloid cells (Supplementary Fig. We also verified that CB2 did not regulate MGLL expression in MC-38 TAMs (Supplementary Fig. The pellets were resuspended in 5 ml of ACK Lysing Buffer (#C3702, Beyotime, Shanghai, China), kept still for 5 min at room temperature, diluted to 15 ml with 10 ml of DMEM and centrifuged at 1000 × g for 5 min. These cells were collected for further isolation of macrophages and other immune cells. These cells were collected for further isolation of T cells.