Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis


De Silva | Rodrigo Zamith


Activated Th2 cells release IL-31 which stimulate itching by acting on IL-31 receptor A (IL-31RA) expressed on sensory nerve fibers and various immune cells, such as MCs, macrophages, DCs, eosinophils and basophils (20–26). The tissues from the AD-dogs were processed to obtain cryosections. However, since the co-localization study between the anti-CB2R and the -GPR55 antibodies showed that the inflammatory cells expressed both the markers (Supplementary Figure 4), it is plausible that CAL immunoreactive cells may also express CB2R-IR. In the present study, MCs expressed CB2R, GPR55, TRPV1, and TRPA1. The dendritic cells of the AD-dogs, although not differentiable from macrophages (since both cell types express IBA1 in dog skin) (51), showed immunoreactivity for all the receptors studied.

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