A collection of cannabinoid-related negative findings from autaptic hippocampal neurons
Bosquez-Berger
We tested the effect of SGIP1 deletion on CB1 signaling in autaptic cultures. Autaptic hippocampal neurons were cultured as described previously34,35. To address these questions, we tested the efficacy of 1-AG to engage CB1 receptors in autaptic hippocampal neurons, recording from neurons to obtain baseline EPSCs, and then treating the cells with 1-AG at various concentrations (Fig. 1-AG (5 µM) did not significantly inhibit EPSCs in CB1−/− neurons, indicating that 1-AG inhibition was CB1-dependent (Fig. To evaluate the role of SGIP1 in neuronal CB1 function, we examined depolarization induced suppression of excitation (DSE) in neurons cultured from mice lacking SGIP1.
Tags:
- Cannabinoid
- Cannabinoid receptor 1
- Cannabinoid receptor
- Endocannabinoid system
- Synapse
- Cell signaling
- 2-Arachidonoylglycerol
- Excitatory postsynaptic potential
- Anandamide
- Chemical synapse
- Fatty acid-binding protein
- Receptor (biochemistry)
- Allosteric regulation
- Allosteric modulator
- Neurophysiology
- Biochemistry
- Cell biology
- Basic neuroscience research
- Neurochemistry